Paper Type |
: |
Research Paper |
Title |
: |
Serological assays and PCR for detection of Toxoplasma gondii
infection in an ostrich farm at Ismailia Province, Egypt |
Country |
: |
Egypt |
Authors |
: |
El-Madawy S R ; Metawea F Y |
 |
: |
10.9790/2380-0235660  |
ABSTRACT:One hundred and twenty serum samples from ostriches (Struthio camelus) in a commercial farm in
Egypt were tested for anti-Toxoplasma antibodies using ELISA test (IgM and IgG ) and Modified agglutination
test (MAT). Five out of 120 birds (4.2%) were found positive to IgM enzyme immunoassay and 11 birds (9.2%)
were IgG enzyme immunoassay positive. Modified agglutination test detected IgG in 15 birds (12.5%). PCR
amplification of Toxoplasma gondii DNA in the blood of birds was recorded in 9 birds (7.5%). The Cases were
categorized into acute and chronic cases depending on the result of blood-PCR and serological test where the
positive cases for IgG were the most frequent (6.7%). The tissues of five dead birds tested by PCR assay showed
the amplification of DNA fragment of T...........
Key word: Toxoplasma, Ostriches, ELISA, MAT, PCR, Egypt..
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