Volume-4 ~ Issue-2 ~ March - April 2018

Abstract: Background: Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder in which prevalence has been increasing steadily all over the world. Type 2 DM represents a significant global health problem. Metabolically triggered inflammation has been proposed as a key step in the pathogenesis of this disease and accelerate atherosclerosis and premature death in subjects. C-reactive protein is an acute phase reactant protein., which is found elevated and serum zinc levels are lower in T2DM. Objectives: To estimate hsCRP (high sensitivity C reactive protein) and serum zinc levels in cases and controls and to compare and analyze the levels of these parameters between two groups.........

 

Key words: T2DM; hsCRP; serum Zinc ; inflammation ; Oxidative stress.

Abstract: Purpose: Medicinal Plants Like Adhatoda Vasica Nees. Are Used Traditionally In Tripura To Cure Pneumonia, Cough And Cold. Pneumonia Is A Lung Infection With Cough, Fever And Hard Time Breathing. Pneumonia Is Mainly Caused By Bacteria Like Streptococcus Pneumoniae. Moreover, Many Of These Pathogenic Strains Are Showing Acute Resistant To Many Commercially Available Antibiotics Due To The Formation Of Biofilms. One Of The Main Areas Of Modern Day Research In Medical Microbiology Is To Search For Agents That Can Destroy Microbial Biofilms Effectively. Materials And Method: The Present Study Evaluates The Antimicrobial Activity Of Adhatoda Vasica Nees. Extracts And Commercially Available Vasicine Against The Biofilms Of The Bacteria - Streptococcus Pneumoniae And Their Free-Living Forms. The Extraction, Identification And Phytochemical........

 

Key words: A. Vasica Nees, Vasicine, Biofilm, Streptococcus Pneumoniae

Abstract: Myocardial Infarction (MI) is one of the major causes of morbidity and mortality in the world. Dyslipidemia is one of the important risk factor. It initiates atherosclerotic plaque formation, finally resulting in degeneration of endothelial cell function which enhances the coagulability of blood by activation of various factors for which apolipoproteins have been implicated. Apolipoprotein A-1 (apo A1) and apolipoprotein B (apo B) are the protein constituents of high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C), respectively. Apo A-1 and HDL-C are antiatherogenic whereas Apo B and LDL-C are atherogenic............

 

Key words: Coronary heart disease, apolipoprotein, lipid profile, dyslipidemia

Abstract: The pharmacological and cytotoxicity evaluation of natural products, extracted from endangered medicinal plants have been reported in several cases. In the present study an attempt was made to evaluate the ethanol extract of both in vivo grown twigs and in vitro generated plantlets of Oroxylum indicum (L.)Vent. with respect to cytotoxicity against human cervical cancer SiHa cells and liver hepatocellular HepG2 cells. MTT assay measured the level of cytotoxicity of ethanol extract of both samples of in vivo and in vitro sources. In later case, plant tissue culture techniques were employed to raise in vitro generated plantlets, for which the nodal regions of in vitro seedling were taken as explants. MS medium, supplemented with BAP (2.5 μg ml-1) proved better than 2,4-D for shoot initiation........

 

Key words: Apoptosis, Cytotoxicity, HepG2 cells, Natural products, Oroxylum indicum, SiHa cells.

Abstract: Non ionizing radiations like microwaves(MW) have been associated with enhanced production of free radicals like,O2, peroxides and super oxidesradicals. The effects of exposure of a non ionizing radiation [microwave] on the HB, PCV, and RBC of fifteen human blood samples of genotypes AA, SS irradiated at a power density of 60mwcm2 have been investigated using the microwavegenerator model ER66OE (NQ 2XC21744) from Toshiba UK, while readings obtained with the aid of a haematocrit reader' The resultsobtained show that there is decrease in the HB concentration and also decrease in the 90 volume of the PCV after irradiation. Comparism of the percentage difference for pre and post irradiation of HB is 4.5. 13.5 and 4.7% for genotypes AA, AS and SS respectively. This result shows the blood genotypes AS is more fragile and most affected by MW exposure. The results indicates that MW exposure may cause adverse physiological consequences on those exposed to high levels of these radiations especially for prolonged periods.

 

Key words: Microwave, Hemoglobin, PCV, RBC, BloodGenotype.

Abstract: As the recombinant bovine growth hormone (rbGH) has become crucial in galactopoiesis research, the main goal of this study was the in vitro generation of histidine-tagged rbGH proteins using the prokaryotic pET system. The isolation of rbgh genes was carried out by PCR using specific primers and a cDNA template retrotranscribed from a sample of total RNA extracted from the bovine pituitary tissue. The PCR products were cloned into the expression vectors pET28a and pET22b. The genetic construction in pET28a added six-histidine residues at the N-terminus region of the rbGH molecule. The genes inserted in pET22b allowed the obtaining of rbGH protein without histidine residues and another variant with the six-histidine residues at the C-terminus region. Different E. coli strains were transformed with the three genetic constructions........

 

Key words: bovine growth hormone; bovine somatotropin; histidine-tag; pET system.

Abstract: Bioinformatics was applied in the creation and maintenance of a database to store biological information at the beginning of the "genomic revolution", such as nucleotide and amino acid sequences. Development of this type of database involved not only design issues but the development of complex interfaces whereby researchers could both access existing data as well as submit new or revised data. Bioinformatics and computational biology are frequently integrated with experimental studies as well, with bioinformatics emphasizing informatics and statistics, while computational biology emphasizes development of theoretical methods, mathematical modeling, and computational simulation techniques to find solution for genetic problems. Bioinformatics studies include analysis and integration of genomics data, prediction of protein function from sequence, and comparisons of protein ligands to identify target effects of drugs. Computational biology includes simulation of protein motion and folding and how proteins interact with each other..

 

Key words: Bioinformatics, Computational Biology, Information Technology,Human Genome Project

Abstract: Azospirillum spp. are considered to be important plant growth promotiverhizobacteria that can improve the growth and yield of several plant species. A collection of Azospirillum spp.isolates were isolated from different regions in Egypt. They were screened for their ability to produce phytohormones especially (IAA and cytokinins). Four isolates were selected depending on their capabilities in production of both IAA and cytokinins. The four isolates were identified depending on 16srDNA gene sequence. One isolate was selected for induction of mutations (K16) using physical mutagen (U.V. light), chemical mutagens (sodium azide). Five U.V. mutants and five sodium azide were selected and their capabilities in production of IAA and cytokinin were recorded. Filtrated fermented broth of two............

 

Key words: Azospirillum sp., fermented broth, U.V. mutagenesis, sodium azide mutagenesis, SDS PAGE, Paulownia tomentosa, In-Vitro propagation

Abstract: Colostrum is the first milk produced by mammary gland of milk-producing animals soon after parturition and its composition varies considerably with the whole milk. In the present study, bovine colostrum was collected within 12 hours of parturition from a local breed of cow from Chennai city and its microbial and nutritive analysis was carried out. The nutritive analysis was compared with locally sourced bovine whole milk. Proteins were five-fold higher and immunoglobulins (Ig) nearly ten-folds higher in the colostrum sample, indicating its metabolic and protective role for the newborn calf. The average count of total heterotropic bacterial population through standard plate count method was found to be 11,000 colony forming units (cfu)/ml. Bacteriological load on colostrum can impact the bioactive protein quality and can have direct influence on the available Ig for the feeding infant.............

 

Key words: Bovine colostrum, LAB, immunoglobulins, lactose, gut flora propagation