ABSTRACT: Development of inbreds, for selecting as parents for hybridisation, in vitro seed germination and its establishment in Spathoglottis albida Kraenzl. was achieved. The pods obtained through selfing were harvested after 30 days and cultured in different media supplemented with individual and combinations of 6-benzylamino purine (BAP) and 1-naphthalene acetic acid (NAA) Seed germination was higher in MS medium (93%) and lower in Mitra medium (87%). The seedlings were grown well in the MS medium supplemented with 1.5 ppm BAP and 0.5 ppm NAA. The plantlets obtained in this medium were healthy and vigorous with 16.8 cm height at the time of deflasking for hardening. The matured rooted plantlets were acclimatized by using potting mixture at 1:1:1 proportion for hardening. The survived plantlets were transferred to field establishment and its growth was successful.
Keywords: Orchid, Inbreeding, Spathoglottis albida, Seed culture, Pollen, Protocorm.
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