Abstract: Two thionin genes were isolated from Arabidopsis thaliana (AT1G12660 and AT1G12663). The resulted thionin protein has antifungal activity against potato pathogenic fungi like Fusariumsolani and Fusariumoxysporum. These two thionin genes were respectively inserted into pEGAD vector. After that transformed into Agrobacterium tumifaceins GV3101::pMP90 in order to be transformed into the two potato cultivars (lady and spunta) using internodal cutting technique of tissue culture.Expressions of thionin transgenes in the transgenic plants were confirmed by..............
Keywords: Solanumtuberosum, Arabidopsis thaliana, Agrobacterium Transformation, Fusariumsolani, Fusariumoxysporum, Thionin genes
[1] J.E. Edmonds, M.S. Vorontsova, and S. Knapp, Solanaceae, In: Flora of Tropical East Africa, ed. H. Beentje, Royal Botanic Gardens, Kew, 2012.
[2] S.S. Kabeil, S.M. Lashin, M.H. El- Masry, M.A. El-Saadani, M.M. Abd-Elgawad, and A.M. Aboul-Einean,: Potato Brown Rot Disease in Egypt: Current Status and Prospects, American-Eurasian J. Agric. and Environ. Sci., 4 (1), 2008, 44-54.
[3] S.I.A Elawady and A.Y. Abdulkheir, An Economic Study of the Growth Determinants for the Egyptian Potatoes Exports to the Global Market, International Journal of Economics and Finance, 7(7), 2015, 89-97.
[4] H.A.M. Mahgoub, G.S.A. Eisa and M.A.H. Youssef, Molecular, biochemical and anatomical analysis of some potato (Solanumtuberosum L.) cultivars growing in Egypt, Journal of Genetic Engineering and Biotechnology, Elseveir. 13, 2015, 39–49.
[5] M.A. Draper, G.A. Secor and H.A. Lamey, Managements of potato diseases in the home garden. NDSU; 1994, 1-8.